Test Overview
Reverse transcription followed by Polymerase Chain Reaction (PCR) with detection by capillary electrophoresis.
The histologic and immunophenotypic characteristics of desmoplastic small round cell tumor (DSRCT) can overlap with that of other "small round blue cell" tumors. The recurrent t(11;22)(p13;q12) chromosomal translocation is characteristic of DSRCT and leads to fusion of the EWSR1 gene at 22q12 and the WT1 gene at 11p13. The overwhelming majority of DSRCT cases harbor this specific translocation. Because of its high prevalence, the EWSR1/WT1 fusion transcript is a highly sensitive diagnostic marker for DSRCT. Its detection can aid in the diagnosis of these tumors, especially in those with unusual histologic features or atypical presentations. This test qualitatively detects the EWSR1/WT1 fusion transcript in fresh/frozen or formalin-fixed paraffin embedded tissues.
Interpretive report provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Test Details
2 - 7 days
- EWS Chimeric Transcript Detection
- WT-1 Chimeric Transcript Detection
- EWS/WT-1 Desmoplastic Small Round Cell Tumor Translocation Assay
- DSRCT TRANSLOCATION ASSAY
- DSRCT
- MLABEL
- DSRCTS
- DSRCT TRANSLOCATION ASSAY
- EWS/WT1 Translocation
- Desmoplastic Small Round Cell Tumor Translocation Detection
Specimen Requirements
For formalin-fixed, paraffin-embedded tissue, a block containing an area with a high percentage of neoplastic cells (for micro-/macro-dissection) is preferred. Unstained, UNBAKED slides (5-8, 10-micron slides; 10-15 if few neoplastic cells are present) with associated H&E stained slide are also acceptable. Decalcified tissue or other fixatives will be accepted and the assay attempted, however these may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.
Additional Information
By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.